Iron (Fe) Test

Test Overview

An iron test checks the amount of iron in the blood. Iron (Fe) is a mineral needed for hemoglobin, the protein in red blood cells that carries oxygen. Iron is also needed for energy, good muscle, and organ function.

About 70% of the body's iron is bound to hemoglobin in red blood cells. The rest is bound to other proteins, such as transferrin or ferritin, or stored in body tissues. When red blood cells die, their iron is released and carried by transferrin to the bone marrow and to other organs such as the liver and spleen. In the bone marrow, iron is stored and used as needed to make new red blood cells.

The source of all the body's iron is food, such as liver and other meat, eggs, beans, and leafy green vegetables. The body needs more iron at times of growth (such as during adolescence), for pregnancy, during breastfeeding, or at times when there are low levels of iron in the body (such as after bleeding).

The iron test checks the:

• Amount of iron in the blood.
• Amount of iron bound to transferrin in the blood (serum).
• Ability of the blood to bind to iron and carry it around the body. This value is called the total iron-binding capacity (TIBC).
• Percentage of transferrin with iron bound to them. This value is called transferrin saturation.

Why It Is Done

A test for iron is done to:

• Check for too much or too little iron in the blood.
• See if your diet is providing enough iron.
• Find out if treatment to keep the right amount of iron in your blood is working.

How To Prepare

Do not eat or drink (other than water) for 12 hours before the test. You will also need to avoid iron supplements for 12 to 24 hours before your test. Your doctor will give you specific instructions. This test is often done first thing in the morning.

How It Is Done

A health professional uses a needle to take a blood sample, usually from the arm.

How It Feels

When a blood sample is taken, you may feel nothing at all from the needle. Or you might feel a quick sting or pinch.

Risks

There is very little chance of having a problem from this test. When a blood sample is taken, a small bruise may form at the site.

Results

Normal

Each lab has a different range for what's normal. Your lab report should show the range that your lab uses for each test. The normal range is just a guide. Your doctor will also look at your results based on your age, health, and other factors. A value that isn't in the normal range may still be normal for you.

Values that may be checked include serum iron, total iron-binding capacity (TIBC), and transferrin saturation.

High and low values

The values for serum iron, total iron-binding capacity (TIBC), and transferrin saturation are used to see if a low amount of iron in the body is from iron deficiency anemia or another condition. The values are also used to see if a high amount of iron is caused by hemochromatosis or another condition. Other conditions that affect iron, TIBC, and transferrin saturation levels include:

• Hemolytic anemia. This causes a low amount of oxygen-carrying hemoglobin found in red blood cells. The iron levels are usually high.
• Thalassemia. This is a blood disorder that runs in families (inherited). It changes how the body makes hemoglobin. The iron levels are often high, and ferritin levels may be high if the person has had a lot of blood transfusions.
• Cirrhosis. This is a condition that occurs when inflammation and scarring damage the liver.
• Lead poisoning. This develops from months or years of exposure to small amounts of lead in the environment.
• Iron deficiency anemia. This occurs when low iron levels cause a low amount of oxygen-carrying hemoglobin in red blood cells. The iron levels are low, the transferrin saturation is high, and the ferritin level is low.
• Rheumatoid arthritis. This form of arthritis inflames the membranes or tissues lining the joints.
• Overuse of iron supplements.
• Bleeding.
• Kidney failure.
• Severe infection.